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为了研究前列腺素(PGF_(2α))的合成释放机理,作者测定了离体豚鼠子宫表面灌流液中PGF_(2α)、PGE_2、6-oxo-PGF_(1α)和凝血素B_2(TXB_2)的释放量,以及动情周期不同阶段(第五天和第七天)、孕酮、雌二醇,催产素和A23187对以上释放的作用。取体重500～800g处女豚鼠,每天做阴道涂片检查,以角化细胞最多时为动情期第一天,取动情期第七天和第十五天(以下称第七天、十五天)豚鼠断颈处死称重,取右侧子宫角纵切悬于浴槽中,与负荷为2 g的等张杠杆相联,用Krebs液做表面灌流(10ml/分)通气(5%CO_2和95%O_2),每个宫角只做以下两实验中一个,每种处理用8个宫
In order to study the mechanism of the synthesis and release of prostaglandin (PGF 2α), the authors measured the release of PGF 2α, PGE 2, 6-oxo-PGF_ (1α) and thromboxane B 2 (TXB_2) from the guinea pig uterine surface perfusate And the effects of progesterone, estradiol, oxytocin and A23187 on the above release during different stages of the estrus cycle (day five and day seven). Take the weight of 500 ~ 800g virgin guinea pigs, do vaginal smear daily examination to the keratinocytes up to the first day of estrus, take the seventh and fifteenth days of estrus (hereinafter referred to as the seventh day, fifteen days) Guinea pigs were sacrificed and their neck severed. The right uterine horns were hung in the bath and connected to an isometric lever with a load of 2 g. Surface perfusion (10 ml / min) with Krebs fluid (5% CO 2 and 95% O_2), one for each of the following two labs, with 8 for each treatment