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采用双梯度不连续密度梯度离心的方法，由喉鳞状细胞癌组织中分离出肿瘤浸润性淋巴细胞（ｔｕｍｏｒｉｎｆｉｌｔｒａｔｉｎｇｌｙｍｐｈｏｃｙｔｅ，ＴＩＬ），经重组人类白细胞介素－２（ｒｅｔｕｒｎｉｎｔｅｒｌｅｕｋｉｎ－２，ｒＩＬ－２）激活，成为活化的ＴＩＬ细胞。采用￣５１Ｃｒ体外释放的方法，检查活化的ＴＩＬ细胞对自体肿瘤细胞、异体肿瘤细胞的杀伤特性；并观察了ＴＩＬ细胞在裸鼠体内对自体肿瘤细胞生长的抑制作用。结果发现活化的ＴＩＬ细胞对自体肿瘤细胞有较强的杀伤作用，但对异体肿瘤细胞的杀伤作用则较弱。实验还证明，在裸鼠体内，ＴＩＬ细胞能抑制自体肿瘤移植瘤系的生长。由新鲜喉鳞癌肿瘤组织中可成功地分离出ＴＩＬ细胞并可大量增殖，可望成为喉癌患者过继免疫治疗的有效来源。
Tumor infiltrating lymphocytes (TILs) were isolated from laryngeal squamous cell carcinoma by double gradient density gradient centrifugation and activated by recombinant interleukin-2 (rIL-2) , Become activated TIL cells. The cytotoxicity of activated TIL cells on autologous tumor cells and allogeneic tumor cells was examined by in vitro release assay of ~51Cr. The inhibitory effect of TIL cells on the growth of autologous tumor cells in nude mice was also observed. The results showed that activated TIL cells have a strong killing effect on autologous tumor cells, but have a weaker effect on allogeneic tumor cells. Experiments also show that in nude mice, TIL cells can inhibit the growth of autologous tumor xenografts. TIL cells can be successfully isolated from fresh laryngeal squamous cell carcinoma tissues and proliferate in large quantities, which is expected to become an effective source of adoptive immunotherapy in patients with laryngeal cancer.