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利用AFLP分子标记技术对181个中国柱花草胶孢炭疽菌菌株进行了基因组DNA分析。分析结果表明柱花草胶孢炭疽菌株DNA存在显著的多态性。聚类分析结果表明181个中国柱花草胶孢炭疽菌株可划分成4大组,但大多数聚于第Ⅰ和Ⅱ组,而第Ⅰ、Ⅱ组下又分别分为4个亚组,表明中国柱花草胶孢炭疽菌正在出现种内遗传分化。同时实验结果还表明中国菌株存在着广泛的生物多样性和遗传变异性,海南菌株与广东、广西的菌株相比多样性较丰富,致病力更强;而海南菌株又以东方、昌江、中国热带农业科学院儋州所部(简称“两院”)3个地区的菌株多样性最为丰富,毒性谱最广泛,这可能与海南种植柱花草历史相对较长、引种活动和生产活动更为频繁等有关。
Genomic DNA analysis was carried out on 181 Chinese strains of Colletotrichum gloeosporioides using AFLP molecular markers. The results showed that there was a significant polymorphism in DNA of Colletotrichum gloeosporioides. Cluster analysis showed that 181 strains of Colletotrichum gloeosporioides isolates could be divided into four groups, but most of them clustered into groups I and II, while groups I and II were further divided into four subgroups, which indicated that China Colletotrichum gloeosporioides are emerging within the genetic differentiation. At the same time, the experimental results also showed that there is a wide range of biodiversity and genetic variability in Chinese strains. Hainan strains are more diverse and stronger in virulence than those in Guangdong and Guangxi. Hainan strains, in turn, The strains in the three regions of Pizhou Prefecture (referred to as “the two institutes”) of the Academy of Tropical Agricultural Sciences are the most abundant and have the widest spectrum of toxicity. This may be due to the relatively long history of the stems and flowers planted in Hainan and the more introductive and productive activities Frequently related.