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(一)严格检查菌种质量要逐瓶观察,有绿、红、黄、黑等色的菌种不用.扩接母种、原种、栽培种更要注意把关,接种后3、7、15、25～30天检查四次,通过不断淘汰保证菌种纯度.栽培时以选35～45天的壮龄菌种最佳.(二)接种前菌种预处理将菌种放进经消毒的接种箱里,每箱可装30瓶,用高锰酸钾和福尔马林密封消毒20分种,然后在酒精灯火焰上烧棉塞上的杂菌,再用75%酒精消毒瓶壁,最后挖去表层老菌膜,扎好瓶口待用.
(A) Strict inspection of strain quality Bottle-by-bottle observation, there are green, red, yellow, black and other strains of bacteria do not need to expand mother species, the original species, cultivars should pay attention to check, after inoculation 3,7,15 , 25 to 30 days to check four times, through continuous elimination of species to ensure purity when cultivated to choose 35 to 45 days of the best strains of young age. (B) pre-vaccination strain put the strain into the sterilized Inoculation box, each box can hold 30 bottles, sealed with potassium permanganate and formalin disinfection of 20 minutes, and then burn in the alcohol lamp flame on the cotton plug on the bacteria, then 75% alcohol disinfection bottle wall, Finally, dig the surface of the old membrane, tie the bottle ready for use.