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本文研究采用全自动网织红细胞计数仪(R2000系统,TOA医学电子公司).使用荧光染料金胺O(AO)结合于网织红细胞中的RNA,根据它们的荧光强度分出高、中、低三个等级(HFR、MFR、LFR),显示从幼稚到成熟各亚群的百分数。作者试图通过网织红细胞计数及成熟参数的检测,观察全血细胞减少症中不同疾病的差异。对象:正常对照292例,(男186,女106),再障67例z,各种恶性肿瘤骨髓浸润69例,脾亢35例,恶性贫血所致巨幼红细胞型贫血13例。年龄10～81岁,男女之比为121:63。全部病例均经骨髓细胞形态学检查和确诊所需的必要试验,包括特殊细胞化学和免疫细胞化学染
In this paper, automatic reticulocyte counter (R2000 system, TOA Medical Electronics Co., Ltd.) was used in this study.RNAs that bind to reticulocytes using the fluorescent dye Auramine O (AO) were classified into high, medium and low according to their fluorescence intensity Three levels (HFR, MFR, LFR) show the percentage of naive to mature subpopulations. The authors attempted to examine differences in different diseases in pancytopenia by testing reticulocyte count and maturation parameters. PARTICIPANTS: 292 normal controls (male 186, female 106), aplastic anemia 67 cases, bone marrow infiltration 69 cases, hypersplenism 35 cases and megaloblastic anemia caused by pernicious anemia in 13 cases. Age 10 to 81 years old, male to female ratio of 121: 63. All cases were tested by bone marrow morphological examination and necessary tests necessary, including special cytochemistry and immunocytochemistry