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本研究以连作烤烟12年的土壤为材料,分析烤烟连作条件下烤烟根际土壤自毒潜力及土壤物质成分差异,为深入揭示烟草连作障碍提供依据。结果表明,连作条件下,烤烟根际土壤对受体植物莴苣(Lactuca sativa)、烟草(Nicotiana tabacum)具有较强的化感自毒潜力。物质成分分析结果显示,未种植烤烟的对照土壤中存在7种糖类物质、14种酸类物质、16种酯类物质、1种其他类物质;而连作烤烟根际土壤中存在2种糖类物质、3种酸类物质、8种酯类物质、12种其他类物质。与对照相比,连作烤烟根际土壤中存在特有的8种与自毒作用相关的物质,如香草醛、洋地黄毒素、雪松醇、植酮、诱虫烯、β-谷甾醇、胆甾烷醇、胆甾烷-3-酮。
In this study, 12-year-old continuous-cropped flue-cured tobacco was used as material to analyze the potential of soil autotoxicity and the difference of soil material composition in flue-cured tobacco rhizosphere so as to provide basis for further revealing continuous cropping obstacle. The results showed that rhizosphere soil had strong allelochemical potential to Lactuca sativa and Nicotiana tabacum under continuous cropping. The results of material composition analysis showed that 7 kinds of sugars, 14 kinds of acids, 16 kinds of esters and 1 kinds of other substances existed in the control soil without flue-cured tobacco, while there were two kinds of sugars Substances, three kinds of acid substances, eight esters, 12 kinds of other substances. Compared with the control, there were 8 specific autotoxins related substances in rhizosphere soil of continuous cropping tobacco such as vanillin, digitoxin, cedrol, phyton, trap insecticide, β-sitosterol, cholestane Alcohol, cholestan-3-one.