CONFORMATION STUDY OF THE LARGE FRAGMENT OF E. COLI DNA POLYMERASE I BY STM

• 作 者: 要小未 李民乾 徐耀良 顾敏明 胡钧 张兰平 张家骅 陆长德 裘敏燕
• 机 构: Institute of Nuclear Research Shanghai 201800 China,Institute of Nuclear Research Shanghai 201800 C
• 出 处: Nuclear Science and Techniques
• 发表日期: 1992年04期
• 关键词: DNA hydration tunneling graphite maintain ellipsoid paraffin specimen conformati

The large fragment of E.coli DNA polymerase I is imaged by scanning tunneling microscope. The specimen is deposited on highly oriented pyrolytic graphite surface, and then covered with pure paraffin oil in order to maintain hydration of the molecules. Images of the enzyme reveal an ellipsoid shape of 5.5-6.0nm wide and 7.0 -7.5 nm long. The conformation of the enzyme is in agreement with the model derived from X- ray crystallography studies.

The large fragment of E. coli DNA polymerase I is imaged by scanning tunneling microscope. The specimen is deposited on highly oriented pyrolytic graphite surface, and then covered with pure paraffin oil in order to maintain hydration of the molecules. Images of the enzyme reveal an ellipsoid shape of 5.5-6.0 nm wide and 7.0 -7.5 nm long. The conformation of the enzyme is in agreement with the model derived from X- ray crystallography studies.