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1目的 探讨 HBs Ag健康携带者自然转阴后外周血淋巴细胞姐妹染色单体互换 (SCE)频率的变化。 2方法 用常规法培养外周血淋巴细胞并用 5 -溴脱氧尿嘧啶核苷诱导 SCE,用紫外线照射法制备 SCE标本 ,对 2 0例 HBs Ag健康携带者 5年自然转阴后外周血淋巴细胞的 SCE频率进行检测 ,同时将 2 0例 HBs Ag阳性的健康携带者作为配对组 ,35例 HBs Ag阴性的健康人作为正常对照组进行 SCE频率的检测。 3结果 上述 3组间的SCE频率不同 (F =3.40 4,P<0 .0 5 ) ,配对组的 SCE频率明显高于对照组 ,差异有显著性 (q=3.6 98,P<0 .0 5 ) ,而转阴组与配对组、对照组相比差异均无显著性 (q=2 .0 17,1.42 3,P>0 .0 5 )。4结论 机体自身有对 HBV感染所致DNA损伤修复的能力。
1 Objective To investigate the changes of sister chromatid exchange (SCE) frequency of peripheral blood lymphocytes in HBs Ag healthy carriers after natural negative conversion. Methods Peripheral blood lymphocytes were cultured by routine method and SCE was induced by 5 - bromodeoxyuridine. The SCE specimens were prepared by UV irradiation. Twenty - five healthy subjects with HBs Ag were assessed for their spontaneous negative peripheral blood lymphocytes SCE frequency was detected. Meanwhile, 20 healthy HBsAg-positive carriers were used as the matched group and 35 healthy HBsAg-negative healthy controls as the normal control group. 3 Results SCE frequency of the above three groups were different (F = 3.40 4, P <0.05), SCE frequency in the paired group was significantly higher than the control group, the difference was significant (q = 3.698, P <0 .0 5). There was no significant difference between the negative group and the matched group or the control group (q = 2.017, 1.42 3, P> 0.05). 4 Conclusion The body itself has the ability to repair DNA damage caused by HBV infection.