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[目的]建立悬乳剂中咪鲜胺和丙环唑含量的HPLC法快速分析方法。[方法]以SHIMADZU 10-A VP Plus高效液相色谱仪,Kromasil 100-5-C18不锈钢色谱柱的高效液相色谱法测定杀菌悬乳剂中咪鲜胺和丙环唑含量的分析方法,流动相为甲醇-磷酸水溶液(pH值2.5,体积比63∶37);流速1.5 mL/min;柱温:室温(温差变化应不大于2℃);检测波长:230 nm;进样量20μL。[结果]咪鲜胺和丙环唑在1~50 mg/L的范围内线性关系良好(线性相关系数0.99991),平均回收率为99.79%、99.62%,咪鲜胺的RSD为0.0336%,丙环唑的RSD为0.0509%。[结论]方法可同时测定杀菌悬乳剂中咪鲜胺和丙环唑的含量,具有准确、快速、精密度好,简便、方法新颖等优点。
[Objective] To establish a rapid HPLC method for the determination of prochloraz and propiconazole in suspoemulsions. [Method] The contents of prochloraz and propiconazole in bactericidal suspoemulsion were determined by HPLC with SHIMADZU 10-A VP Plus high performance liquid chromatography and Kromasil 100-5-C18 stainless steel column. The mobile phase (PH 2.5, volume ratio 63:37); flow rate 1.5 mL / min; column temperature: room temperature (temperature difference should not exceed 2 ℃); detection wavelength: 230 nm; injection volume 20μL. [Results] The prochloraz and propiconazole had a good linearity (linear correlation coefficient of 0.99991) in the range of 1 ~ 50 mg / L with the average recoveries of 99.79% and 99.62%. The RSD of prochloraz was 0.0336% The RSD for the prazole is 0.0509%. [Conclusion] The method can simultaneously determine the contents of prochloraz and propiconazole in bactericidal suspoemulsion with the advantages of accuracy, rapidness, good precision, simplicity, novel method and so on.