论文部分内容阅读
Aim:To examine the effects of novel peroxisome proliferator-activated receptor(PPAR)α/γ dual agonist C333H on insulin resistance and lipid metabolism.Methods:An established dual-luciferase reporter gene assay system was used invitro to test the activity of C333H with respect to the transcription of humanPPARα and PPARγ.A preadipocyte differentiation assay and reverse transcrip-tion-polymerase chain reaction were used to detect the functional activities ofC333H.In db/db mice,the effects of C333H were investigated with respect tolowering of blood glucose and lipid levels.Results:C333H was determined to bea novel PPARα/γ dual agonist because it strongly induced luciferase activity onhuman PPARα and PPARγ,promoting the differentiation of preadipocytes toadipocytes,and functioning in upregulating the expression of some glucose andlipid metabolic target genes of the PPAR.In addition,C333H efficiently reducedblood lipid and glucose concentrations in db/db diabetic mice.Conclusion:C333Hhas dual action on both PPARα and PPARγ,and might be of interest for theamelioration of lipid metabolic disorders and insulin resistance associated withtype 2 diabetes.
Aim: To examine the effects of novel peroxisome proliferator-activated receptor (PPAR) alpha / gamma dual agonist C333H on insulin resistance and lipid metabolism. Methods: An established dual-luciferase reporter gene assay system was used in invitro to test the activity of C333H with respect to the transcription of human PPARα and PPARγ. A preadipocyte differentiation assay and reverse transcrip-tion-polymerase chain reaction were used to detect the functional activities of C333H.In db / db mice, the effects of C333H were investigated with respect tolowering of blood glucose and lipid levels. Results: C333H was determined to bea novel PPARα / γ dual agonist because it strongly induced luciferase activity onhuman PPARα and PPARγ, promoting the differentiation of preadipocytes to adipocytes, and functioning in upregulating the expression of some glucose and lipid metabolic target genes of the PPAR .In addition, C333H highly reduced blood lipid and glucose concentrations in db / db diabetic mice. Confluence: C333 Hhas dual action on both PPARα and PPARγ, and might be of interest for the gameioration of lipid metabolic disorders and insulin resistance associated with type 2 diabetes.