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抗癌药敏试验的方法学研究对肿瘤化疗用药个体化.提高疗效.减轻毒副作用具有现实意义.近年来.检测细胞线粒体琥珀酸脱氢酶活性以反映细胞存活和增殖情况的MTT法引起广泛关注。本文以人早幼粒白血病HL—60细胞为对象.观察MTT法检测细胞存活、增殖情况和该法对5种抗癌药物敏感性的检测结果与台盼兰拒染法、~3H—TdR掺入法的—
The methodological study of anticancer drug susceptibility testing is of great importance to the individualization of chemotherapy drugs for cancer treatment, improving the curative effect and alleviating the toxic and side effects .In recent years, MTT assay to detect mitochondrial succinate dehydrogenase activity to reflect cell survival and proliferation has caused widespread attention. In this paper, human promyelocytic leukemia HL-60 cells as the object.Observed MTT assay cell survival and proliferation and the method of the five anti-cancer drug sensitivity test results and trypan blue exclusion method, ~ 3H-TdR doping Into the law -