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随机将１７４只大鼠分为Ⅰ、Ⅱ、Ⅱ组，每组５８只动物，Ⅰ组：用生理盐水灌胃；Ⅱ组：用毒蕈液灌胃；Ⅲ组：毒蕈液灌胃同时腹腔注射维生素Ｅ。观察肾脏生化和形态学改变，Ⅱ组灌胃后６小时，大鼠肾皮质脂质过氧化物含量显著增高，过氧化氢酶和超氧化物歧化酶活力显著降低；１２小时后大鼠肾皮质钠钾ＡＴＰ酶活性显著降低；Ⅱ组灌胃后６小时光、电镜下大鼠肾近曲和远曲小管上皮细胞变性，４８小时肾间质淋巴细胞浸润，Ⅲ组动物生化和形态学损害显著轻于Ⅱ组，表明自由基损伤效应在毒蕈急性肾损伤的发病机理中起着重要作用。
174 rats were randomly divided into Ⅰ, Ⅱ, Ⅱ groups, each group of 58 animals, Ⅰ group: with saline gavage; Ⅱ group: poisonous mushrooms intragastric administration; Ⅲ group: poisonous mushroom liquid intragastric administration at the same time Vitamin E injection The renal biochemical and morphological changes were observed. After 6 hours of intragastric administration, the content of lipid peroxidation in renal cortex and the catalase and superoxide dismutase activity of rats in group Ⅱ were significantly decreased. After 12 hours, Na + K + ATPase activity was significantly decreased in group Ⅱ. Six hours after intragastric administration, the renal proximal and distal convoluted tubule epithelial cells were degenerated by electron microscopy and the interstitial infiltration of renal interstitium was observed in 48 hours. The biochemical and morphological changes were significant in group Ⅲ Lighter than the group Ⅱ, indicating that the free radical damage effect plays an important role in the pathogenesis of acute mushroom injury.