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目的对成年颞叶癫大鼠进行丰富环境干预,探讨丰富环境是否能促进认知功能以及是否对齿状回的神经发生产生影响。方法将成年Wistar大鼠随机分为4组:假手术组、丰富环境+假手术组、癫组、丰富环境+癫组。丰富环境干预30 d后,应用Morris水迷宫实验进行学习记忆功能评价,并应用免疫荧光技术观察海马齿状回新生神经细胞的情况。结果一般线性重复变量分析示逃避潜伏期[F(3,36)=9.810,P<0.05)]、上台前路程[F(3,36)=11.092,P<0.05)],各组间差异有显著性;而游泳速度差异无显著性[F(3,36)=0.776,P>0.05)]。丰富环境+假手术组海马齿状回DCX阳性细胞(新生神经细胞标志物)[(65.00±6.10)个]显著多于假手术组[(32.43±2.76)个](P<0.05),癫组DCX阳性细胞[(21.30±2.97)个]较假手术组明显减少(P<0.05);丰富环境+癫组DCX阳性细胞[(51.50±3.16)个]明显多于癫组(P<0.05)。结论丰富环境有利于成年颞叶癫大鼠认知功能的恢复。
Objective To investigate the effects of enriched environment on cognitive function and the neurogenesis of dentate gyrus in rats with temporal lobe epilepsy. Methods Adult Wistar rats were randomly divided into 4 groups: sham operation group, rich environment + sham operation group, epilepsy group, rich environment + epilepsy group. After 30 days of environmental intervention, Morris water maze test was used to evaluate the learning and memory function, and the hippocampal dentate gyrus neurons were observed by immunofluorescence. Results The general linear repeat variables analysis showed that the escape latency [F (3,36) = 9.810, P <0.05), the distance before going to the station [F (3,36) = 11.092, P <0.05) Significant; however, there was no significant difference in swimming speed [F (3, 36) = 0.776, P> 0.05)]. Compared with the sham operation group [(32.43 ± 2.76)] (P <0.05), epilepsy was significantly (P <0.05) positive in DC + positive cells (21.30 ± 2.97) DCX positive cells were significantly decreased (P <0.05). Compared with the sham operation group, the number of DCX positive cells (51.50 ± 3.16) in the enriched environment + epilepsy group was significantly higher than that in the epilepsy group (P < 0.05). Conclusion Enrichment environment is beneficial to the recovery of cognitive function in adult temporal lobe epileptic rats.