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为了解螺旋粉虱Aleurodicus dispersus体内细菌多样性和主要优势菌群结构,用PCR-DGGE和16S rRNA文库对采自于海南省番石榴上螺旋粉虱雌、雄成虫体内的细菌群落进行了分析。用PCR扩增体内细菌16S rRNA基因,构建雌、雄虫克隆文库;再用限制性片段长度多态性(restriction fragment length polymorphism,RFLP)方法从文库中筛选不同16S rRNA基因图谱,根据图谱对克隆子进行分型。从螺旋粉虱雌、雄两个样品中共获得10种分类操作单元(operational taxonomic unit,OTUs)。以16S rRNA基因为基础构建系统发育树,系统发育分析表明,螺旋粉虱雌、雄成虫体内优势菌群主要为发酵菌属Zymobacter,杀雄菌属Arsenophonus,泛菌属Pantoea和假单胞菌属Pseudomonas。Candidatus Portiera aleyrodidarum和Arsenophonus sp.可能为其体内共生菌群,在所有样品中均可稳定地检测到。这些微生物可能对螺旋粉虱生长发育、繁殖和性比调控起到重要的协同作用。
In order to understand the bacterial diversity and dominant flora in Aleurodicus dispersus, the bacterial community in female and male adults collected from Guava Whiteflies in Hainan Province was analyzed by PCR-DGGE and 16S rRNA libraries. The bacterial 16S rRNA gene was amplified by PCR, and the female and male clones were constructed. The different 16S rRNA gene maps were screened from the library by restriction fragment length polymorphism (RFLP) Sub-sub-type. A total of 10 operational taxonomic units (OTUs) were obtained from the female and male of the B. spiralis. The phylogenetic tree was constructed based on 16S rRNA gene. Phylogenetic analysis showed that the predominant colonies of female and male adult Leptospira interdictus were mainly Zymobacter, Arsenophonus, Pantoea and Pseudomonas Pseudomonas. Candidatus Portiera aleyrodidarum and Arsenophonus sp. May be their commensal flora in vivo and are detected stably in all samples. These microorganisms may play an important synergistic effect on the growth, reproduction and sexual regulation of B. tabaci.