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目的建立柱前衍生化HPLC/UV法测定黄杨宁片中环维黄杨星D的含量。方法异氰酸苯酯为柱前衍生化试剂,流动相:甲醇-水(83∶17);ZORBAX SB-C18(250 mm×4.6 mm,3.5μm)柱分离;检测波长242 nm;流速:1 mL.min-1;柱温:30℃;进样量:10μL。结果环维黄杨星D在2~400μg.mL-1与峰面积呈良好线性关系,回归方程为:Y=2 616X+198.2(r=0.999 9);环维黄杨星D平均回收率为102.6%,RSD为0.4%(n=6);结论该法简单、准确度高、灵敏度好,为黄杨宁片质量控制提供了依据。
Objective To establish a precolumn derivatization HPLC/UV method for the determination of Cyclovirobuxine D in Huangyangning tablets. Methods Phenyl isocyanate was used as pre-column derivatization reagent. The mobile phase consisted of methanol-water (83:17); ZORBAX SB-C18 (250 mm×4.6 mm, 3.5 μm) column separation; detection wavelength 242 nm; mL.min-1; column temperature: 30°C; injection volume: 10 μL. Results There was a good linear relationship between Cyclovirobuxine D and its peak area at 2~400μg.mL-1. The regression equation was: Y=2 616X+198.2 (r=0.999 9). The average recovery rate of Cyclovirobuxine D was 102.6%. RSD was 0.4% (n=6). Conclusions This method is simple, accurate, and sensitive, providing a basis for quality control of Huang Yang Ning.