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固体法生产白僵菌,一般要经过一、二、三级培养,生产时期长,数量大,操作比较繁重,技术掌握不好,易造成杂菌污染。为简化白僵菌生产方法,1976年我们初步试验成功了玉米心叶繁菌治螟法,进行了防治玉米螟的田间试验;1977年我们又在45亩的玉米田进行了防治示范,另外,牡丹江地区林口县新城公社、合江地区防治示范面积各约1万亩,都获得了良好效果。这种方法,就是用二级母剂接种三级原料后,不经过室内培养,直接撒在玉米心叶里,使白僵菌在玉米心叶中自然繁殖,达到治螟目的。
Beauveria bassiana is produced by the solid method, generally through one, two and three levels of cultivation, the production period is long, the number of large, more complicated operation, poor technical control, easy to cause bacterial contamination. In order to simplify the production of Beauveria bassiana, in 1976 we successfully tested the corn leaf heartbeat method for borer control, and conducted field trials on preventing and controlling corn borer. In 1977, we conducted demonstration on prevention and treatment of 45-acre corn field. In addition, Mudanjiang Linkou County Metro commune, Hejiang area prevention and control demonstration area of about 1 million mu, have achieved good results. This method is to use two levels of mother liquor inoculation of three raw materials, without the indoor culture, sprinkled directly in the corn heart leaves, Beauveria bassiana in the natural propagation of corn leaf to achieve the purpose of the treatment of stem borer.