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目的研究氯化消毒饮用水有机提取物对SD大鼠睾丸结构及精子质量的影响。方法于2011年7—9月(丰水期)采集贵阳市某地水厂的管网末梢水水样。将40只SD雄性大鼠随机分为4组,分别为溶剂对照组(予玉米油)及3、15、75 L/kg饮用水有机提取物染毒组,每组10只,采用灌胃方式进行染毒,每天1次,连续染毒4周。末次染毒24 h后,对大鼠的精子计数、精子活动度及精子形态进行测定。结果与对照组比较,75 L/kg组大鼠睾丸生精小管直径、间质细胞数、精子计数均较低,空泡状生精小管比率、生精细胞脱落比率、空泡状生精细胞比率以及生精细胞脂滴发生率均较高;15、75 L/kg组大鼠睾丸生精细胞核异常率和精子畸形率均较高,精子活动度较低,差异有统计学意义(P<0.05)。结论该地较高剂量的氯化消毒饮用水有机提取物染毒可引起SD大鼠睾丸组织损伤和精子质量降低。
Objective To study the effect of chlorinated disinfection drinking water organic extract on testicular structure and sperm quality in SD rats. Methods From July to September 2011 (Fengshui period), water samples were collected from the pipe network at a waterworks site in Guiyang. Forty male Sprague-Dawley rats were randomly divided into four groups: the solvent control group (corn oil) and the organic extract of 3,15,75 L / kg drinking water, 10 rats in each group. To be infected, once daily, for 4 weeks. After the last 24 h exposure, sperm count, sperm motility and sperm morphology of rats were determined. Results Compared with the control group, the testicular seminiferous tubule diameter, number of interstitial cells and sperm count in 75 L / kg group were lower, the ratio of vacuolar spermatogenic cells, spermatogenic cell shedding ratio, vacuolar spermatogenic cells The rate of spermatogenic cells lipid droplets and the incidence were higher; 15,75 L / kg group spermatogenic cell nucleus abnormalities and sperm deformity rate were higher sperm motility was lower, the difference was statistically significant (P < 0.05). Conclusion The high dose of chlorinated disinfection drinking water organic extract can cause damage of testicular tissue and sperm quality in SD rats.