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采用温室盆栽法,研究了银胶菊的花水浸提液对小麦生长及其生理生化机制的影响,以明确其对小麦的化感作用。结果显示,花水浸提液抑制小麦种子萌发和幼苗生长,且抑制强度随浸提液浓度的升高而加强;随着花水浸提液浓度的增加,小麦叶片光合作用、光合色素含量和过氧化氢酶(CAT)活性下降,磷(P)和丙二醛(MDA)含量升高,氮(N)含量呈现先降低后趋于正常的变化趋势,硝酸还原酶(NR)活性呈现先降低后升高的变化趋势,谷氨酰胺合成酶(GS)活性没有显著变化,而过氧化物酶(POD)和超氧化物歧化酶(SOD)活性则呈现先升高后降低的变化趋势。表明银胶菊的花水浸提液抑制小麦叶片光合作用,提高了小麦叶片的MDA含量,并降低了小麦叶片SOD、POD和CAT的活性。
The greenhouse pot method was used to study the effects of the aqueous extract of guayule on wheat growth and its physiological and biochemical mechanisms to clarify its allelopathic effects on wheat. The results showed that the flower water extract inhibited wheat seed germination and seedling growth, and inhibitory strength with the concentration of the extract increased and strengthened; With the concentration of water extract increased, the photosynthesis of wheat leaves, photosynthetic pigment content and Catalase (CAT) activity decreased, P and MDA contents increased, while N content decreased first and then tended to normal. Nitrate reductase (NR) (P <0.05) and the activity of superoxide dismutase (SOD) showed the trend of first increasing and then decreasing. The results showed that the aqueous extract of guayule inhibited the photosynthesis of wheat leaves, increased the MDA content of wheat leaves and decreased the activities of SOD, POD and CAT in wheat leaves.