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胆囊胆汁用无水乙醇处理,38±2℃水浴吹干后,1.0g/L BSA40倍稀释(A—B)法,可较好地去除胆汁中抑制物对癌胚抗原(Carcinoembryonic antigen,CEA)测定的干扰.重复实验和回收实验结果表明,此法重复5～6次预处理胆汁样品后,变异系数7.3%～11.8%,回收率为56.7%～91.3%,比不经处理胆汁样品直接加样测定,检出值高出4～19倍.对临床结直肠肿瘤及早期小的隐匿性肝转移具有较高诊断价值.胆囊胆汁CEA测定A—B法预处理时间短,方法简便,是一般实验室都能开展的一种实用可靠的检测方法.
Gallbladder bile with ethanol treatment, 38 ± 2 ℃ water bath after drying, 1.0g / L BSA40-fold dilution (A-B) method can be better to remove bile inhibitors on carcinoembryonic antigen (CEA) The results of repeated experiments and recovery experiments showed that the method of this method repeated 5 to 6 times of bile samples pretreatment, the coefficient of variation of 7.3% ~ 11.8%, the recovery rate was 56.7% ~ 91.3%, compared with untreated bile samples directly Like test, the detection value higher than 4 to 19 times for clinical colorectal tumors and small early occult liver metastases have a higher diagnostic value of gallbladder bile CEA determination of A-B method of pre-treatment time is short, the method is simple Laboratory can carry out a practical and reliable detection methods.