论文部分内容阅读
应用ABC染色法在进行石蜡切片单克隆抗体筛选过程中,常会遇到如下问题,①因需低温保存组织中的抗原成份,故需在低温37℃烤干切片,如何使其切片在染色中不脱片。②单克隆抗体染色的温度与时间。③如何减少背景非特异性染色。④染色方法。就这些问题浅谈点滴体会。
In the process of paraffin-embedded monoclonal antibody screening by ABC staining, we often encounter the following problems: ① Because of the need to preserve the antigenic components in tissues at low temperature, it is necessary to dry the slices at a low temperature of 37 ° C and how to slice them in the stain Take off. ② monoclonal antibody staining temperature and time. How to reduce background non-specific staining. ④ staining method. On these issues talk about bit by bit experience.