论文部分内容阅读
80年代兴起的脑活体微电极伏安法具有组织损伤小,定位准确,快速灵敏等优点,并可连续测定,有时程慨念,对研究神经递质的快速释放过程和传导机理有特殊的价值,在动物脑内,大部分神经递质与其代谢产物,以及一些神经肽化合物都可以氧化,采用电化学方法测定在理论上是可行的。但是为了提高灵敏度和消除干扰,Gonon等首先提出将碳纤维电极预先经频率为70Hz交流三角波处理,再采用示差脉冲伏安法测定或采用带有特殊扫描波
The voltammetry of living microelectrodes in the 1980s has the advantages of small tissue damage, accurate positioning, quick and sensitive detection, and can be continuously measured and time-laden. It has special value in studying the rapid release process and conduction mechanism of neurotransmitters. Animal brain, most of the neurotransmitter and its metabolites, as well as some neuropeptide compounds can be oxidized, the use of electrochemical determination is theoretically feasible. However, in order to improve the sensitivity and eliminate the interference, Gonon et al first proposed that the carbon fiber electrode be pre-processed by an AC triangular wave at a frequency of 70 Hz, and then measured by differential pulse voltammetry or by using a special scanning wave