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目的探究二甲双胍对多囊卵巢综合征卵巢血管生成的作用。方法利用脱氢表雄酮构建大鼠多囊卵巢综合征模型,检测分析大鼠卵巢形态变化、血管生成因子表达和血清胰岛素水平。结果 16天后,二甲双胍组体重略有下降(P<0.05)。PCOS组和二甲双胍组卵巢重量比对照组有所增加(P<0.05),但二甲双胍组的卵巢重量及卵巢策量/体重值较PCOS组降低(P<0.05)。PCOS组大鼠卵巢早期窦状卵泡含量与对照组基本相同,但是二甲双胍治疗后的大鼠卵巢早期窦状卵泡显著增加(P<0.01)。PCOS组大鼠胰岛素水平比对照组高3倍左右(P<0.01);二甲双胍治疗后,大鼠胰岛素浓度大幅降低,与对照组浓度相似(P>0.05)。PCOS组大鼠卵巢的内皮细胞面积较对照组明显增多(P<0.01),二甲双胍治疗后,大鼠卵巢内皮细胞面积显著减小(P<0.01)。PCOS大鼠血液中VEGF量比对照组明显增高(P<0.05),二甲双胍治疗后的大鼠血液VGEF含量明显降低(P<0.05)。结论二甲双胍不仅能调节多囊卵巢综合征大鼠卵巢血管内皮生长因子的水平和改善卵泡的发育形态,还可以调节卵巢血管生成,促进卵泡发育和排卵。
Objective To investigate the effect of metformin on ovarian angiogenesis in patients with polycystic ovary syndrome. Methods Deoxyepiandrosterone was used to construct the model of polycystic ovary syndrome in rats. The morphological changes of ovaries, expression of angiogenic factors and serum insulin were detected and analyzed. Results After 16 days, body weight in metformin group decreased slightly (P <0.05). The ovary weight in PCOS group and metformin group was higher than that in control group (P <0.05), but the weight of ovary and ovulation / body weight in metformin group were lower than those in PCOS group (P <0.05). The content of early sinusoidal follicles in ovary of rats in PCOS group was basically the same as that in control group, but the early sinusoid follicles in ovariectomized rats were significantly increased after metformin treatment (P <0.01). The insulin level in rats in PCOS group was about 3 times higher than that in control group (P <0.01). After metformin treatment, the insulin concentration in rats was significantly lower than that in control group (P> 0.05). The area of endothelial cells in PCOS group was significantly higher than that in control group (P <0.01). After metformin treatment, the area of ovarian endothelial cells was significantly reduced (P <0.01). The level of VEGF in the blood of PCOS rats was significantly higher than that of the control group (P <0.05), and the content of VGEF in the blood of rats treated with metformin was significantly lower (P <0.05). Conclusions Metformin can not only regulate the level of ovarian vascular endothelial growth factor and improve the development of ovarian follicles in polycystic ovary syndrome rats, but also regulate ovarian angiogenesis and promote follicular development and ovulation.