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目的 检测正常人群血浆中 TAFI:Ag和 TAFI:A的水平 ,确立正常人血浆中 TAFI:Ag和TAFI:A的参考值范围及其在冠心病患者血浆中的变化。方法 应用 ELISA方法测定 34名正常人、1 9例冠心病患者及 1 5例 DIC患者血浆中 TAFI:Ag的含量 ,应用发色底物法检测血浆中 TAFI:A的水平。对正常人、冠心病患者血浆中 TAFI:Ag和 TAFI:A的含量进行对照分析。结果 正常人 TAFI:Ag平均值为 ( 77± 2 8) % ,参考值范围为 2 1 %～ 1 33% ,TAFI:A平均值为 ( 2 4± 5 ) μg/ml,参考值为 1 4～ 34μg/ml。冠心病患者血浆中 TAFI:Ag为 ( 1 5 6± 32 ) % ,TAFI:A为 ( 4 3± 7) μg/ml。结论 正常人血浆中的TAFI:Ag含量具有很大变异性 ,正常人 TAFI的活性与 TAFI抗原含量呈剂量相关性 ,冠心病患者血浆中 TAFI:Ag和 TAFI:A显著增高 ,表明 TAFI在冠心病发病中起一定作用。
Objective To detect the levels of TAFI: Ag and TAFI: A in plasma of normal people and to establish the reference value range of TAFI: Ag and TAFI: A in plasma of normal people and their changes in plasma of patients with coronary heart disease. Methods The plasma levels of TAFI: Ag in 34 normal subjects, 19 coronary heart disease patients and 15 DIC patients were measured by ELISA. The level of TAFI: A in plasma was measured by chromogenic substrate method. The plasma levels of TAFI: Ag and TAFI: A in normal subjects and patients with coronary heart disease were analyzed. Results The average value of TAFI: Ag was (77 ± 28)% in normal subjects, the range of reference value was 21% ~ 33%, the average of TAFI: A was (24 ± 5) μg / ml and the reference value was 14 ~ 34 μg / ml. Plasma TAFI: Ag was (165 ± 32)% and TAFI: A was (43 ± 7) μg / ml in patients with coronary heart disease. CONCLUSION TAFI: Ag in normal human plasma has great variability. The activity of TAFI in normal human is dose-dependently correlated with TAFI antigen content. TAFI: Ag and TAFI: A are significantly increased in plasma of patients with coronary heart disease. The incidence plays a role.