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目的采用动物实验研究的方法,探讨鱼精蛋白在心肌缺血-再灌注期的低血压反应及其机理。方法20只雄性家兔,随机分为二组,对照组(A组)、实验组(B组),建立在体心肌短暂缺血(15min)-再灌注(60min)损伤模型,A组于前降支阻断前20min给予肝素(700U/kg)。B组于前降支阻断前20min给予肝素(700u/kg),再灌注25min给予鱼精蛋白以拮抗肝素。于缺血前、再灌注期间,取右房血检测NO、MDA水平,测定血流动力学指标变化及心肌组织学观察。结果B组于再灌注25min给予鱼精蛋白后,MAP较A组明显下降(P<0.05),伴随血清NO显著增高(P<0.05)。MDA及组织学检查两组无显著差别。结论鱼精蛋白参与心肌缺血-再灌注期低血压反应的发生,其机理可能于促使内皮细胞NO的大量产生有关。
Objective To investigate the hypotensive response and its mechanism of protamine during myocardial ischemia-reperfusion in animal experiments. Methods Twenty male rabbits were randomly divided into two groups: control group (group A) and experimental group (group B), which were established in the model of myocardial ischemia (15min) - reperfusion (60min) Heparin (700 U / kg) was given 20 minutes before the descending branch was blocked. In group B, heparin (700u / kg) was given 20 minutes before the anterior descending artery occlusion, and protamine was administered for 25 minutes to reperfusion heparin. During ischemia and reperfusion, right atrial blood was taken to measure NO and MDA levels, and the changes of hemodynamics and myocardial histology were observed. Results Compared with group A, MAP significantly decreased (P <0.05) and serum NO was significantly increased (P <0.05) in group B at 25 min after reperfusion. MDA and histological examination showed no significant difference between the two groups. Conclusion Protamine is involved in the occurrence of hypotensive reaction during myocardial ischemia / reperfusion. Its mechanism may be related to the massive production of NO in endothelial cells.