论文部分内容阅读
目的 :研究咽喉部恶性黑色素瘤多药耐药 (MDR)基因胎盘型谷胱甘肽 S 转移酶 (GST π)、DNA拓朴酶Ⅱ (TopoⅡ )和MDR基因编码产物P 糖蛋白 (Pgp)的表达及其与预后的关系。 方法 :应用链霉素亲生物素 过氧化物酶标S P法检测 2 8例咽喉部恶性黑色素瘤患者Pgp、GST π和TopoⅡ的表达 ,分析MDR基因及MDR基因编码产物阳性表达率与肿瘤主要临床病理特征及预后的关系。结果 :2 8例标本中Pgp、GST π和TopoⅡ的表达率分别为 35 .7%、5 7.1%和 4 6 .4 % ,相互间差异无显著性意义 (P >0 .0 5 )。Pgp、GST π和TopoⅡ的表达与性别、年龄、肿瘤大小及Clark’s分级、Breslow’s分级无明显相关 (P >0 .0 5 ) ,与AJC分级及预后显著相关 (P <0 .0 5 )。结论 :Pgp、GST π和TopoⅡ等多因素联合作用是咽喉部恶性黑色素瘤多药耐药的主要作用机制 ,与预后相关。检测MDR基因及其编码产物在咽喉部恶性黑色素瘤化疗敏感性预测中具有必要性和可行性
Objective: To investigate the relationship between placental glutathione S-transferase (GST π), Topo Ⅱ (MDR) gene and Pgp gene of MDR gene in pharyngeal laryngeal mucosa Expression and its relationship with prognosis. Methods: The expression of Pgp, GST π and Topo Ⅱ in 28 cases of throat malignant melanoma was detected by streptavidin-peroxidase-labeled SP method. The positive rates of MDR gene and MDR gene were analyzed and compared with those of tumor Relationship between pathological features and prognosis. Results: The expression rates of Pgp, GST π and Topo Ⅱ in 28 samples were 35.7%, 51.1% and 46.4%, respectively. There was no significant difference between them (P> 0.05). The expression of Pgp, GST π and Topo Ⅱ was not significantly correlated with gender, age, tumor size, Clark’s grade and Breslow’s grade (P> 0.05), but significantly correlated with grade and prognosis of AJC (P <0.05). Conclusion: The combined effect of Pgp, GST π and Topo Ⅱ is the main mechanism of multidrug resistance in malignant melanoma of the throat, which is related to the prognosis. Detection of MDR Gene and Its Coding Product Necessity and Feasibility in Predicting Chemosensitivity of Malignant Melanoma in the Throat