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由于烟草的分子标记开发和遗传图谱构建十分困难,迄今烟草中有关数量性状基因座(QTL)的定位研究仍非常有限。本研究利用一个由207个株系组成的烤烟DH群体及基于该群体所构建的含有24个连锁群、611个SSR标记,总长为1882.1cM的遗传图谱,采用复合区间作图方法,对株高(PH)、茎围(SG)、节距(IL)、叶片数(LN)、最大腰叶长(LWL)和最大腰叶宽(WWL)6个与叶片产量有关的农艺性状进行QTL定位分析。共检测到69个QTL,大部分QTL的效应值较小,仅有4个具有较大的效应值,可解释大约15%~20%的表型变异。6个性状之间大多彼此相关。与此相符,在基因组中发现存在许多小区域,每个区域包含2个或2个以上紧密连锁的不同性状的QTL。
Due to the difficulty of molecular marker development and genetic map construction in tobacco, the location of QTLs in tobacco has so far been limited. In this study, a 207-line flue-cured tobacco DH population and a genetic map based on the population of 24 linkage groups and 611 SSR markers with a total length of 1882.1 cM were constructed. The composite interval mapping QTL mapping was conducted on 6 agronomic traits related to leaf yield, including PH, SG, IL, LN, LWL and WWL . A total of 69 QTLs were detected, most of the QTLs had smaller effect values, and only 4 had larger effect values, accounting for about 15% to 20% of phenotypic variation. Most of the 6 traits are related to each other. Consistent with this, many small regions are found in the genome, and each region contains two or more closely linked QTLs with different traits.