论文部分内容阅读
目的:建立HPLC-DAD同时测定苦碟子注射液中的7种黄酮类成分[木犀草素-7-O-龙胆二糖苷(LGT)、木犀草素-7-O-β-D-吡喃葡萄糖苷(LGCOP)、木犀草素-7-O-β-D-吡喃葡萄糖醛酸苷(LGCRP)、芹菜素-7-O-β-D-吡喃葡萄糖苷(AGCOP)、芹菜素-7-O-β-D-吡喃葡萄糖醛酸苷(AGCRP)、木犀草素(LI)、芹菜素(AGI)]含量的方法。方法:采用Waters Symmetry C18色谱柱(3.9 mm×150 mm,4.6μm),流动相0.05%甲酸-乙腈和0.05%甲酸-水,梯度洗脱。流速1 mL·min-1,柱温35℃,检测波长340 nm。结果:7种待测成分的分离度良好,线性关系良好,加样回收率为99.0%~101.5%,均符合含量测定要求。建立的方法能够同时测定LGT,LGCOP,LGCRP,AGCOP,AGCRP,LI,AGI的含量,并对8批苦碟子注射液进行含量测定,各黄酮含量在0.157 9~103.4 mg·L-1。结论:该法简便可行,结果可靠,且能同时测定苦碟子注射液中7中黄酮类成分,可作为本制剂多成分内控质量的测定方法。
OBJECTIVE: To establish a HPLC-DAD method for the simultaneous determination of seven flavonoid components [Luteolin-7-O-gentiobioside (LGT), luteolin-7-O-β-D-pyran Glucoside (LGCOP), luteolin-7-O-β-D-glucopyranoside (LGCRP), apigenin-7-O-β-D-glucopyranoside (AGCOP) 7-O-β-D-glucopyranoside (AGCRP), luteolin (LI) and apigenin (AGI) Methods: The gradient was performed on a Waters Symmetry C18 column (3.9 mm × 150 mm, 4.6 μm) with a mobile phase of 0.05% formic acid-acetonitrile and 0.05% formic acid-water. The flow rate was 1 mL · min-1, the column temperature was 35 ℃ and the detection wavelength was 340 nm. Results: The separation of 7 kinds of tested components was good and the linearity was good. The recoveries of samples ranged from 99.0% to 101.5%, which met the requirements of content determination. The established method can simultaneously determine the contents of LGT, LGCOP, LGCRP, AGCOP, AGCRP, LI and AGI, and determine the content of each batch of Kudinzi injection. The content of each flavone is between 0.157 9 and 103.4 mg · L -1. Conclusion: The method is simple and feasible, the results are reliable, and can simultaneously determine the content of flavonoids in Kudiezi injection 7, which can be used as the determination method of multi-component internal control quality of the preparation.