论文部分内容阅读
为观察重组ＰｘＴ１－Ｐ５３在真核细胞中的表达及其与Ｆａｓ表达的关系，笔者采用磷酸钙—ＤＮＡ共沉淀法，ＤＥＡＥ—莆聚糖介导的ＤＮＡ转染法将ＰｘＴ１－Ｐ５３转移至ＰＡ３１７细胞内，并用抗Ｐ５３和抗Ｆａｓ抗体，经间接免疫荧光法证实转染ＰｘＴ１－Ｐ５３的ＰＡ３１７细胞不仅能表达Ｐ５３蛋白，而且亦能表达Ｆａｓ抗原。本实验结果表明笔者构建的ＰｘＴ１－Ｐ５３确实能在真核细胞内表达，并提示Ｐ５３可能通过其转录因子活性，诱导Ｆａｓ等基因表达，以阻止不能修复的已损伤ＤＮＡ的细胞发生恶性转化
To observe the expression of recombinant PxT1-P53 in eukaryotic cells and its relationship with Fas expression, PxT1-P53 was transferred to PA317 by calcium phosphate-DNA co-precipitation method and DEAE-PPS mediated DNA transfection Intracellular, and with anti-P53 and anti-Fas antibodies, confirmed by indirect immunofluorescence PA317 cells transfected with PxT1-P53 not only express P53 protein, but also express Fas antigen. The experimental results show that the PxT1-P53 constructed by the author can indeed be expressed in eukaryotic cells and suggest that P53 may induce the expression of Fas and other genes through its transcription factor activity to prevent malignant transformation of the irreparable DNA damaged cells