论文部分内容阅读
为了从分子水平上明确近年来我国苹果生产上发生的A、B、D和F 4种不同症状型苹果果实轮纹病的遗传分化现象及其不同致病菌的遗传变异程度。本研究对来自我国三大苹果产区的26个相关病原菌株的DNA指纹图谱进行了分析(Table 1)。结果表明 (1) 上述4种不同症状型轮纹病原菌株在基因组DNA水平上具有明显的遗传差异(Fig.1&Table 2),并在所分析的11个多态性引物中有4个引物的扩增图谱(Table 4)均可使上述4种不同致病型菌株相互区别(Table 5)。统计分析结果显示,这4种不同致病菌相互之间的遗传距离为0.1314~0.2541(Table 6);(2) 相同症状型的不同来源致病菌之间具有较高的遗传相似性 (88.4~100%)(Table 3),由此说明苹果果实轮纹病菌的遗传分化与地理环境条件差异无明显的相关性。 以上研究结果对于进一步阐明苹果果实轮纹病害的遗传演化规律及该病害监测与防治技术的改进具有重要的理论与实际意义。
In order to clarify the genetic differentiation of apple rings disease and the genetic variation degree of different pathogenic bacteria in apple fruit of 4 different symptom types A, B, D and F occurred in apple production in China in recent years. In this study, DNA fingerprinting of 26 related pathogenic strains from three major apple producing areas in China was analyzed (Table 1). The results showed that: (1) The 4 different symptom type rotifers showed obvious genetic differences at the genomic DNA level (Fig.1 & Table 2), and among the 11 polymorphic primers analyzed, 4 primers Table 4 distinguishes the above 4 different pathogenic strains from each other (Table 5). The statistical analysis showed that the genetic distances among the four different pathogenic bacteria were 0.1314 ~ 0.2541 (Table 6). (2) The genetic similarity among different pathogenic bacteria with the same symptom was high (88.4 ~ 100%) (Table 3), indicating that there is no obvious correlation between the genetic differentiation of apple fruit ring rot and the difference of geographical environment. The above results have important theoretical and practical significance for further clarifying the law of genetic evolution of apple tree rot disease and the improvement of the disease monitoring and control technology.