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Let σ be an automorphism of a semiprime ring R, and let f, g, h, k be maps from a set S into R. The identity f(s)xg(t) = h(s)σ(x)k(t) for s, t ∈ S and x ∈ R is characterized. As an application, the form of σ-biderivations on semiprime rings is determined.