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研究了用毛细管区带电泳法快速测定复方新诺明片中磺胺甲口恶唑 ( SMZ)和甲氧苄氨嘧啶 ( TMP)的含量。使用长 2 7cm(有效长度 2 0 cm)内径 50 μm的石英毛细管 ,2 5k V分离电压 ,在 0 .0 5mol/L的乙酸盐缓冲液 ( p H5.5)中 ,上述两组分可在 1 .5min内完全分离。用紫外检测器在 2 1 4nm处检测 ,外标法定量。 1 0次测定含有 1 41 .7mg/L SMZ和 2 5.6mg/L TMP的试样溶液 ,相对标准偏差为 1 .0 % ( SMZ)和 0 .8% ( TMP) ,回收率为 98.0 % ( SMZ)和 1 0 1 .0 % ( TMP) ,检出限 ( 3σ)分别为 3.5mg/L( SMZ)和 1 .1 mg/L( TMP)。建立的方法测定复方新诺明片剂中两组分的含量 ,所得结果与药典法结果一致
The rapid determination of sulfamethoxazole (SMZ) and trimethoprim (TMP) in cotrimoxazole tablets by capillary zone electrophoresis was studied. Using a 2 7 cm (effective length 20 cm) quartz capillary tube with an internal diameter of 50 μm, a 25 kV separation voltage was used, and in 0.050 mol / L acetate buffer (p H5.5) Complete separation in 1.5 minutes. Detected with UV detector at 214nm, external standard method. The sample solutions containing 1 41.7 mg / L SMZ and 2 5.6 mg / L TMP were determined 10 times with relative standard deviations of 1.0% (SMZ) and 0.8% (TMP) and a recovery of 98.0% ( The limits of detection (3σ) were 3.5 mg / L (SMZ) and 1.1 mg / L (TMP), respectively. The established method for the determination of the content of the two components in the cotrimoxazole tablets, the obtained results are consistent with the results of the pharmacopoeia