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为了建立一种猪伪狂犬病gB抗体检测试纸,为养猪业基层工作人员提供一种快速简便的狂犬病免疫抗体评价方法。胶体金标记gB蛋白作为探针,SPA和猪抗伪狂犬病病毒多抗IgG分别作为检测线和质控线,建立了猪伪狂犬病gB抗体检测试纸。猪伪狂犬病质控血清和疫苗免疫猪血清用来评价其特异性,敏感性以及与IDEXX PRV gB ELISA试剂盒的符合率试验。试验结果显示,该试纸条具有较高的特异性和敏感性,与IDEXX PRV gB ELISA试剂盒的符合率为91.04%;该免疫层析试纸条具有快速(5min)、特异、敏感等优点,不需要专门的仪器设备和专业技术人员,适合于田间推广应用。
In order to establish a test strip of PRV gB antibody, it provides a quick and easy method to evaluate rabies immune antibody for the grass-roots staff in pig industry. Colloidal gold labeled gB protein as a probe, SPA and porcine anti-pseudorabies virus polyclonal anti-IgG as a test line and quality control lines, the establishment of the PRV gB antibody test strip. Porcine PRV quality control serum and vaccine-immunized swine serum were used to assess their specificity, sensitivity, and coincidence rate with the IDEXX PRV gB ELISA kit. The test results showed that the strip has high specificity and sensitivity, and the coincidence rate with IDEXX PRV gB ELISA kit was 91.04%. The immunochromatographic strip has the advantages of fast (5min), specificity and sensitivity , Do not need specialized equipment and professional and technical personnel, suitable for field promotion and application.