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目的 :测定烧伤患者肾脏透析排出液中头孢他啶的含量。方法 :采用高效液相色谱法 ,色谱柱 :μ Bondapak -C18 柱 ;流动相 :甲醇 -醋酸胺 (17∶83 ,V/V ) ;流速 :1 0ml/min ;检测波长 :254nm。结果 :头孢他啶在0 5～100μg/ml的范围内线性关系良好 ,r=0 9999 ;日内、日间RSD≤3 ,平均回收率100 30 %。结论 :本法简便、快速、准确
Objective: To determine the content of ceftazidime in renal dialysis effluent of burn patients. Methods: High performance liquid chromatography (HPLC) was used. The column was: μ Bondapak -C18 column; the mobile phase was methanol-acetic acid amine (17:83, V / V); the flow rate was 10 ml / min; the detection wavelength was 254 nm. Results: Ceftazidime showed a good linearity in the range of 0 5 ~ 100 μg / ml with r = 0 9999. The intra-day and inter-day RSD≤3 and the average recovery was 100 30%. Conclusion: This method is simple, fast and accurate