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目的用大鼠Walker-256移植性肝癌模型研究癌痛消颗粒对大鼠肝癌miRNAs的调节作用,以探讨癌痛消颗粒抗肝癌的可能机制。方法将60只大鼠移植性肝癌模型随机分为5组,即癌痛消颗粒高剂量组(A组)、中剂量组(B组)、低剂量组(C组)、5-Fu组(D组)、生理盐水组(E组),于造模后第8天对癌痛消治疗组及生理盐水组灌胃给药,5-Fu组腹腔注射给药,连续用药14 d后停药24 h,处死大鼠,取出瘤块,用实时定量聚合酶链反应(qRT-PCR)技术观察肝癌特定miRNAs(miR-199a-3p、miR-199a-5p、miR-18)表达水平。另设正常饲养组(F组)大鼠10只,不进行造模及给药,供实验结束时作空白对照用。结果造模组与空白对照组比较miR-18有更高水平的表达,miR-199a-3p,miR-199a-5p表达水平降低;造模各组(A、B、C、D组)药物干预后与生理盐水组(E组)比较肝癌组织miR-18表达水平降低,miR-199a-3p,miR-199a-5p表达水平升高(P<0.01);和E组比较,A、B、C、D组均可明显调节miRNAs水平,癌痛消中剂量组比5-FU组更为明显(P<0.01)。结论口服癌痛消颗粒对大鼠移植性肝癌组织的miRNAs表达具有一定的调控作用,提示癌痛消的抗癌机制可能与调控肝细胞癌发生和发展相关的miRNAs水平有关。
OBJECTIVE: To study the regulatory effect of Jiantongxiao Granule on rat liver cancer miRNAs by using rat Walker-256 transplanted liver cancer model in order to explore the possible mechanism of Jiantongxiao Granule in inhibiting liver cancer. Methods Sixty rat liver transplantation models were randomly divided into five groups: high-dose group (A group), middle-dose group (B group), low-dose group (C group) and 5-Fu group D group) and saline group (E group). The rats in Jiaotong eliminating group and NS group were intragastrically administrated on the 8th day after model making. The rats in 5-Fu group were injected intraperitoneally and discontinued after 14 days After 24 h, the rats were sacrificed and the nodules were removed. The expression of miR-199a-3p, miR-199a-5p and miR-18 in hepatocellular carcinoma was detected by real-time quantitative polymerase chain reaction (qRT- Another normal feeding group (F group) rats 10, not modeling and administration for the end of the experiment for blank control. Results The expression of miR-199a-3p and miR-199a-5p in model group was higher than that in blank control group Compared with the saline group (E group), the expression of miR-18 in miR-199a-3p and miR-199a-5p groups were significantly decreased (P <0.01) , And group D could obviously regulate the level of miRNAs. The dose of Jie Tong Xiao Zhong was obviously more than that of 5-FU group (P <0.01). Conclusions Oral Gu Tong Xiao particles can regulate the expression of miRNAs in rat liver allografts, suggesting that the anticancer mechanism of Jie Tong Xiao may be related to the regulation of miRNAs related to the occurrence and development of hepatocellular carcinoma.