论文部分内容阅读
目的:研究苦参素空间稳定脂质体的制备方法并考察其药剂学性质。方法:采用正交设计筛选处方,乙醇注入法制备苦参素空间稳定脂质体;用葡聚糖凝胶G-50柱分离空间稳定脂质体和游离药物,用HPLC法测定包封率;用透射电镜观察空间稳定脂质体的外观形态,并用粒径分析仪测定空间稳定脂质体的粒径和Zeta电位;进一步考察空间稳定脂质体的释放规律。结果:所得空间稳定脂质体的包封率为(85.39±1.21)%;形态为粒径均匀的球形和类球形,粒径为(156±10)nm,Zeta电位为(-39.0±3.06)mV;空间稳定脂质体的体外释放符合Higuchi方程;具有较好的稳定性。结论:优选得到的空间稳定脂质体处方和制备工艺合理、稳定,其体外释放具有缓释特点。
OBJECTIVE: To study the preparation of liposomes with stable kualin and to investigate its pharmacological properties. METHODS: Orthogonal design was used to screen prescription and oxytocin injection to prepare space-stabilized oxymatrine liposomes. The liposomes and free drug were separated by Sephadex G-50 column, and the encapsulation efficiency was determined by HPLC. The morphology of the sterilized liposomes was observed by transmission electron microscopy. The size and Zeta potential of the sterilized liposomes were determined by particle size analyzer. The release of sterically stabilized liposomes was further investigated. Results: The entrapment efficiency of liposomes was (85.39 ± 1.21)%. The morphology of the liposomes was spherical and spheroidal with the diameter of (156 ± 10) nm and the zeta potential of (-39.0 ± 3.06) mV. The in vitro release of sterically stable liposomes is in accordance with the Higuchi equation and has good stability. Conclusion: The optimized formulation and preparation of sterilized liposomes are reasonable and stable, and their in vitro release has the characteristics of sustained release.