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[目的 ]研究中药瑞香素在体外和体内的杀裂殖体作用。 [方法 ]在恶性疟原虫FCCl株常规体外培养中测试瑞香素杀裂殖体活性 ,并按“四天抑制性试验”在感染伯氏疟原虫ANKA株的小鼠中测定不同剂量瑞香素的体内抗疟活性。 [结果 ]体外试验中瑞香素在 1～ 10 μmol L剂量范围内有明显杀灭裂殖体作用 ,而体内试验中按D4减虫率与感染鼠在 30d内的平均生存天数评价 ,5 0或 10 0mg kg .d- 1 × 4d瑞香素灌胃以及 10 ,5 0或 10 0mg kg.d- 1 × 4d瑞香素腹腔注射给药在伯氏鼠疟原虫ANKA株感染鼠中的抗疟作用与 10mg kg .d- 1 × 4d氯喹 (CQ)灌胃的疗效相似。 [结论 ]瑞香素在体外和体内均有一定的杀裂殖体作用。
[Objective] To study the role of Chinese medicine daphnetin in killing schizont in vitro and in vivo. [Method] The dSabin activity was tested in routine in vitro culture of Plasmodium falciparum FCCl strain. The daphnetin in vivo was tested in mice infected with Plasmodium berghei ANKA strain according to the “four-day inhibitory test” Anti-malarial activity. [Result] In vitro, daphnetin could obviously kill schizont in the dose range of 1 ~ 10 μmol L, while in vivo test was based on the evaluation of the D4 reduction rate and the mean survival time of infected mice within 30 days, Anti-malarial effect of intraperitoneal injection of 10 0 mg kg .d-1 × 4 d daphnetin and 10, 50, or 100 mg kg.d-1 × 4 d daphnetin in mice infected with ANKA strain of Plasmodium berghei 10mg kg. D - 1 × 4d chloroquine (CQ) gavage similar efficacy. [Conclusion] Daphnetin has a certain schizophrenia effect both in vitro and in vivo.