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目的研究海藻溴酚化合物对糖尿病肾损伤大鼠结缔组织生长因子(CTGF)蛋白表达的影响。方法采用STZ注射制作糖尿病大鼠模型,随机分为对照组、模型组、化合物A和化合物B剂量组(0.5,10mg.kg-1),灌胃给药12周。处死大鼠,测血浆及肾组织中谷胱甘肽过氧物酶(GSH-Px)的活力及丙二醛(MDA)的含量;透射电镜法观察大鼠肾组织的病理改变;免疫组化法检测CTGF蛋白的表达。结果与对照组相比,模型组血浆及肾组织匀浆中GSH-Px活力下降,MDA含量升高。各干预组中GSH-Px的活力较模型组有升高的趋势,MDA含量有下降趋势。各干预组的CTGF平均吸光度值低于模型组。电镜下各干预组肾小球及肾小管病变较模型组减轻。结论海藻溴酚化合物A、B能提高糖尿病大鼠机体抗氧化水平,并能一定程度改善肾脏的病理变化,但其具体机制有待进一步探讨。
Objective To study the effect of bromophenols from seaweed on the expression of connective tissue growth factor (CTGF) in rats with diabetic nephropathy. Methods STZ-induced diabetic rat models were randomly divided into control group, model group, compound A and compound B dose groups (0.5, 10 mg.kg-1), and intragastric administration for 12 weeks. The rats were sacrificed and the activities of glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in plasma and kidney tissues were measured. The pathological changes of renal tissues were observed by transmission electron microscopy. CTGF protein expression was detected. Results Compared with the control group, the activity of GSH-Px in plasma and renal homogenate of model group decreased and MDA content increased. The activity of GSH-Px in each intervention group was higher than the model group, MDA content decreased. The average CTGF absorbance value of each intervention group was lower than that of the model group. Under electron microscope, glomerular and tubular lesions in each intervention group were alleviated compared with model group. Conclusion The alginate-bromophenol compounds A and B can improve the antioxidant status of diabetic rats and can improve renal pathological changes to a certain extent, but the specific mechanism remains to be further explored.