论文部分内容阅读
目的探讨新型肝穿刺方法建立原发性肝多房棘球蚴(Echinococcus multilocularis,E.m)小鼠模型。方法将120只12w龄健康雌性小鼠随机分为切开皮肤经腹壁肌层肝穿刺接种、开腹肝穿刺接种和经皮肝穿刺接种3组,每组40只。20%乌拉坦腹腔注射麻醉后,分别对3组小鼠进行肝脏注射E.m组织混悬液,制备模拟原发性肝多房棘球蚴小鼠模型。结果 3组小鼠的存活率依次为85%、75%、80%(P>0.05),肝脏E.m感染率依次为70.6%、70.0%、62.5%(P>0.05),胸腔误穿率依次为0%、0%、25%(P<0.01)。结论切开皮肤经腹壁肌层肝穿刺接种可成功制备模拟原发性肝多房棘球蚴小鼠模型,既简化了开腹接种的操作,又减少了经皮肝穿刺误穿胸腔的发生率。
Objective To explore a new liver puncture method to establish a mouse model of primary liver Echinococcus multilocularis (E.m.). Methods A total of 120 12-day-old healthy female mice were randomly divided into three groups (40 in each group). 20% of urethane anesthesia after intraperitoneal injection, respectively, three groups of mice were injected E. coli liver tissue suspension to prepare mice model of simulated primary liver polyketideria. Results The survival rates of mice in three groups were 85%, 75% and 80% (P> 0.05), and the rates of hepatic Em infection were 70.6%, 70.0% and 62.5% (P> 0.05) 0%, 0%, 25% (P <0.01). Conclusions The model of mouse model of H. parvum is successfully prepared by incision of the skin through the liver puncture in the abdominal wall, which not only simplifies the operation of laparotomy but also reduces the incidence of percutaneous transhepatic puncture in the chest .