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目的:观察参芪扶正注射液对免疫低下小鼠免疫功能的调节作用以及核因子-kappaB(NF-κB)和IκBα蛋白表达的影响。方法:将48只C57BL/6小鼠随机分为空白对照组、模型组、参芪扶正注射液低剂量组和高剂量组,每组12只。除空白对照组外,各组小鼠给予环孢素A诱导建立免疫低下模型,同时参芪扶正注射液低、高剂量组分别每天腹腔注射参芪扶正注射液15 mL/kg、30 mL/kg,连续给药14 d。第15天小鼠禁食麻醉后,取血离心取血清,采用ELISA法检测各组小鼠血清中白细胞介素-2(IL-2)和γ-干扰素(IFN-γ)水平。取脾脏称重计算脾脏指数,检测脾脏中IL-2、IFN-γ的表达,以及脾脏组织中NF-κB和IκBα蛋白的表达。结果:与模型组比较,参芪扶正注射液低、高剂量组脾脏指数有显著的升高(P<0.05);与模型组比较,血清、脾脏中的IL-2、IFN-γ在参芪扶正注射液低、高剂量组中显著升高(P<0.05);与模型组比较,参芪扶正注射液低、高剂量组脾脏细胞核内的NF-κB蛋白表达显著升高;细胞质内的IκBα蛋白表达明显降低。结论:在免疫低下小鼠模型中,参芪扶正注射液能够通过提高脾脏指数、升高IL-2、IFN-γ的表达,调节NF-κB、IκBα蛋白的表达,以达到提高机体免疫功能的目的。
Objective: To observe the regulation effect of Shenqi Fuzheng Injection on immune function and the expression of NF-κB and IκBα protein in immunocompromised mice. Methods: 48 C57BL / 6 mice were randomly divided into blank control group, model group, Shenqi Fuzheng injection low dose group and high dose group, 12 rats in each group. Except for the blank control group, mice in each group were induced with cyclosporine A to establish an immunosuppressive model. At the same time Shenqifuzheng injection low and high dose groups were injected intraperitoneally with Shenqi Fuzheng Injection 15 mL / kg, 30 mL / kg , Continuous administration of 14 d. On the 15th day, the mice were fasted and anesthetized. Blood was collected by centrifugation and the levels of interleukin-2 (IL-2) and interferon-γ (IFN-γ) in serum of each group were detected by ELISA. Spleen weight was taken to calculate the spleen index. The expression of IL-2 and IFN-γ in spleen and the expression of NF-κB and IκBα in spleen were detected. Results: Compared with the model group, the spleen index of Shenqi Fuzheng injection group was significantly increased (P <0.05). Compared with the model group, the serum and spleen IL-2 and IFN- Compared with the model group, the expression of NF-κB in the nucleus of spleen of low and high dose Shenqifuzheng injection group was significantly increased; the expression of IκBα in cytoplasm Protein expression was significantly reduced. CONCLUSION: Shenqi Fuzheng injection can increase the expression of IL-2 and IFN-γ and increase the expression of NF-κB and IκBα protein in the immunosuppressive mouse model in order to improve immune function purpose.