Immunosensor application based on Raman spectroscopy of porous silicon

• 作 者: 江涛 莫家庆 吕小毅 晏鹏飞 贾振红 李江伟 张富春
• 机 构: School of Physics, Xinjiang University,School of Electronic and Information Engineering, Xi'an Jiaot
• 出 处: Chinese Optics Letters
• 发表日期: 2011年02期
• 关键词: inexpensive covalent ultimate linker protective label Controlled steps linearly

Porous silicon (PS) suitable for optical detection of immunoreaction is fabricated. The structure of immunosensor is prepared by the following steps: oxidization, silanization, glutaraldehyde cross-linker, and covalent binding of antibody. When antigen is added into the immunosensor, the Raman intensity is estimated to be linearly reduced according to the concentration of the surface protective antigen protein A (spaA) of below 4.0 μg ml-1. The ultimate detection limit is 1.412 × 102 pg ml-1. Controlled experiments are also presented with non-immune antigen of the spaA, and results show that the immunosensor has high specificity. Compared with the conventional enzyme-linked immuno sorbent assay (ELISA), this method is quick, inexpensive, and label-free.

Porous silicon (PS) suitable for optical detection of immunoreaction is fabricated. The structure of immunosensor is prepared by the following steps: oxidization, silanization, glutaraldehyde cross-linker, and covalent binding of antibody. When antigen is added into the immunosensor, the Raman The ultimate detection limit is 1.412 × 102 pg ml-1. Controlled experiments are also presented with non- immune antigen of the spa A, and results show that the immunosensor has high specificity. Compared with the conventional enzyme-linked immuno sorbent assay (ELISA), this method is quick, inexpensive, and label-free.