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肿瘤分子生物学研究结果表明,多种肿瘤细胞过度表达表皮生长因子受体(EGFR);体外和动物研究证实,抗EGFR单抗可显著抑制一些过度表达EGFR的癌细胞的生长,这是因为抗EGFR单抗竞争性地抑制EGF或TGF-α与EGFR的结合.从而阻断了酪氨酸激酶介导的细胞信号传递而发挥其抑制癌细胞生长作用.由于EGFR是一种膜抗原,且在非小细胞肺癌中过度表达,因而抗EGFR单抗的免疫交联物在非小细胞肺癌抗体药物导向治疗中有可能具有潜在的应用价值.在本研究中我们观察了抗EGFR单抗-丝裂霉素结合物对人肺腺癌裸鼠皮下种植瘤的抗癌作用,对抗EGFR单抗在非小细胞肺癌抗体导向化学治疗中的应用价值进行了初步探讨.方法:取8周龄裸鼠10只,每组5只分为治疗组和对照组.用人肺腺癌SPC-Al细胞株(10~7)接种裸鼠左腋窝皮下,
Tumor molecular biology studies have shown that a variety of tumor cells overexpress epidermal growth factor receptor (EGFR); in vitro and animal studies confirmed that anti-EGFR monoclonal antibody can significantly inhibit the growth of some cancer cells overexpressing EGFR because of the resistance The EGFR monoclonal antibody competitively inhibits the binding of EGF or TGF-α to EGFR, thereby blocking tyrosine kinase-mediated cell signaling and exerting its effect of inhibiting cancer cell growth. Since EGFR is a membrane antigen, it is Over-expression in non-small cell lung cancer, so immune cross-linked anti-EGFR monoclonal antibody may have potential application value in non-small cell lung cancer antibody-oriented therapy. In this study we observed the anti-EGFR monoclonal antibody - mitosis The anticancer effect of conjugates on human lung adenocarcinoma in nude mice implanted in nude mice and the application value of anti-EGFR monoclonal antibody in non-small cell lung cancer antibody-directed chemotherapy. Methods: Take 8 weeks old nude mice 10 Only 5 mice in each group were divided into treatment group and control group. Human lung adenocarcinoma SPC-Al cell line (10~7) was used to inoculate subcutaneously in the left armpit of nude mice.