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利用电导(EL)法和电阻抗图谱(EIS)法对脱锻炼期间欧美杨‘107杨’(Populus×canadensis Moench cv.‘Neva’)×大青杨(P.ussuriensis Kom.)F1子代进行抗寒性测定,试图通过比较两种方法测定抗寒性结果的相关性,以确定EIS法快速测定‘107杨’×大青杨F1子代抗寒性的最佳参数。以8个杂种子代和母本‘107杨’3年生为试材,进行了电阻抗图谱法和电导法分析,结果表明:脱锻炼期间,各子代抗寒性均逐渐降低,依次为NK36>NK41>NK61>NK57>NK42>NK60>NK58>NK56,均高于‘107杨’,EIS参数弛豫时间[EIS(τ)法]和EL法的抗寒性测定结果间存在明显的线性相关(R2=0.965),但EIS法测定的抗寒性低于EL法,且未经冷冻处理样本的胞外电阻率(re)和弛豫时间(τ)与经冷冻处理EL测定的抗寒性有较高的相关性,R2分别为0.817和0.847。
The F1 progenies of Populus × canadensis Moench cv. ’Neva × P. ussuriensis Kom. Were treated with EL and EIS methods In order to determine the best parameters of the ’107 Yang’ × Populus euchromatii F1 progenies cold resistance by EIS method, the cold resistance was determined by comparing the two methods. The results of electrical impedance spectroscopy and conductivity analysis showed that the cold resistance of each progeny of 8 hybrid progenies and their female parent ’107 Yang’ was reduced gradually, followed by NK36 NK41> NK61> NK57> NK42> NK60> NK58> NK56, both were higher than those of ’107 Yang’. There was a significant linear correlation between the EIS parameter relaxation time [EIS (τ) (R2 = 0.965), but the cold resistance measured by the EIS method was lower than that of the EL method, and the extracellular resistivity (re) and relaxation time (τ) of the non-frozen samples were significantly lower than those of the frozen-processed EL There is a high correlation, R2 = 0.817 and 0.847, respectively.