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鸟氨酸氨基甲酰移换酶(OCT)主要存在于哺乳动物肝脏内,小肠按单位重量计算所合的OCT活力约为肝脏的1/10(人类),而其他组织及体液仅含微量。例如健康人类的血清中,用一般的测定方法几乎检查不出来,不少文献指出:当人类患了伴着肝细胞损伤的疾患时,血清OCT的活力大增,可作为诊断肝脏功能的指标。但对于在肝损伤时,肝脏本身OCT活力的变化如何?到目前为止,尚鲜报导。本实验系观察大白鼠在四氯化碳中毒及以3′甲基-4-二甲基氨基偶氮苯(3′-Me-DAB)诱发肝癌癌变过程中肝脏OCT活力的变化。OCT活力的测定系按前文所报导的根据产物氨的生成量为指标,以1克新鲜肝组织在
Ornithine carbamoyltransferase (OCT) is mainly present in the liver of mammals. The OCT activity of the small intestine per unit weight is about 1/10 of that of the liver (human), while other tissues and body fluids contain only trace amounts. For example, the serum of healthy human beings can hardly be detected by ordinary methods. Many literatures point out that when human beings suffer from the diseases with liver cell damage, the activity of serum OCT greatly increases and can be used as an index to diagnose liver function. But what about changes in liver OCT activity at liver injury? So far it has not been reported yet. This experiment was to observe the change of hepatic OCT activity in rats with carbon tetrachloride poisoning and carcinogenesis induced by 3’-methyl-4-dimethylaminoazobenzene (3’-Me-DAB) in rats. Determination of OCT activity according to the previously reported based on the amount of ammonia produced as an indicator to 1 g of fresh liver tissue in