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最近的体外研究表明某些T4抗原决定簇可能起着AIDS病毒受体的作用。本文报道了用AIDS病人末梢血(PBL)冰冻切片鉴别HTLV-Ⅲ抗原的方法。该方法对于流行病学研究及AIDS病人化疗的追踪研究可能是十分重要的。 PBL采集于患AIDS的18名婴儿及42名成人。血液经聚蔗糖泛影葡胺分层,分离的2×10~6个淋巴细胞沉积物包埋于0.2ml 1%的琼脂糖内,迅速置液氮内,然后进行冰冻切片。切成的1～2μm厚的片子经3次固定后存于-70℃冰箱待测。感染HTLV-Ⅲ
Recent in vitro studies indicate that certain T4 epitopes may play a role as AIDS virus receptors. This article reports a method for the identification of HTLV-III antigens using frozen sections of AIDS patients peripheral blood (PBL). This method may be very important for the follow-up of epidemiological studies and chemotherapy of AIDS patients. PBL was collected from 18 infants with AIDS and 42 adults. Blood was separated by Ficoll diatrizoate, and 2 × 10 ~ 6 lymphocyte sediments were embedded in 0.2 ml of 1% agarose, rapidly placed in liquid nitrogen, and frozen sectioned. Cut into 1 ~ 2μm thick film after 3 times stored in -70 ℃ refrigerator to be measured. Infection with HTLV-III