论文部分内容阅读
目的:研究不同的表面活性剂的细胞毒性,从而筛选出低毒性的表面活性剂,为以后的进一步研究表面活性剂逆转肿瘤细胞的多药耐药提供基础。方法:采用MTT(四甲基偶氮唑盐)检测方法,研究9种不同类型表面活性剂对肿瘤细胞K562／S及其多药耐药株K562／A02的细胞毒性,并比较同一表面活性剂对药物敏感肿瘤细胞和多药耐药肿瘤细胞的细胞毒性之间的差别。结果:非离子表面活性剂对肿瘤细胞K562／S及其多药耐药细胞K562／A02的IC50在10-1mg·ml-1数量级,阳离子及阴离子表面活性剂的IC50为10-3 mg·ml-1数量级。结论:非离子表面活性剂的细胞毒性最小,阴离子表面活性剂次之,阳离子表面活性剂的细胞毒性最大,同一表面活性剂对药物敏感肿瘤细胞及其肿瘤耐药细胞的细胞毒性没有差异,表明表面活性剂对肿瘤细胞多药耐药的逆转不是由其本身的细胞毒性引起的。
OBJECTIVE: To study the cytotoxicity of different surfactants to screen out low-toxicity surfactants and provide a basis for further studies on the reversal of multi-drug resistance of tumor cells by surfactants. Methods: MTT assay was used to study the cytotoxicity of nine different types of surfactants on K562 / A and multidrug resistant K562 / A02 cells. The cytotoxicity of the same surfactant Differences in cytotoxicity between drug-sensitive tumor cells and multidrug-resistant tumor cells. Results: The IC50 of nonionic surfactants on K562 / S and multidrug-resistant cells K562 / A02 was in the range of 10-1 mg · ml-1. The IC50 of the cationic and anionic surfactants was 10-3 mg · ml -1 order of magnitude. CONCLUSION: Non-ionic surfactants have the lowest cytotoxicity, followed by anionic surfactants and cationic surfactants with the highest cytotoxicity. The same surfactant has no difference in cytotoxicity to drug-sensitive tumor cells and tumor-resistant cells The reversal of multidrug resistance of tumor cells by surfactants is not caused by their own cytotoxicity.