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目的观察急性百草枯(PQ)中毒大鼠肾脏病理变化和肾脏caspase-3的表达,探过PQ中毒肾损伤病理生理机制。方法 36只健康SD大鼠随机分为对照组和染毒组,染毒组合予80 mg/kg PQ一次性灌胃染毒建立急性中毒模型,对照组等量生理盐水灌胃。分别观察24、48、72 h肾组织病理学变化,同时采用免疫组织化学法观察caspase-3蛋白表达。计量资料以均数±标准差(x±s)表示,组间单因素方差分析,结果以P<0.05为差异有统计学意义。结果对照组肾组织结构清晰,染毒组肾组织结构清晰度明显下降,染毒24 h可见充血、水肿等病理变化,随时间延长而加重;肾小球亦可受累。对照组caspase-3蛋白多不表达;染毒组染毒24 h在皮质部肾小管上皮细胞的胞膜及胞浆中caspase-3蛋白呈阳性表达,免疫组织化学评分(IHS)升高,与对照组各时间点比较,差异均有统计学意义(P<0.05)。结论 caspase-3蛋白高表达提示其可能参与PQ中毒肾损伤病理生理过程。
Objective To observe the renal pathological changes and the expression of caspase-3 in rats with acute paraquat (PQ) poisoning and to explore the pathophysiological mechanism of renal injury induced by PQ poisoning. Methods Thirty-six healthy SD rats were randomly divided into control group and exposure group. Acute poisoning model was established by intraperitoneal injection of 80 mg / kg PQ once a day. The rats in control group were given normal saline. The pathological changes of kidney were observed 24, 48 and 72 h respectively, and the expression of caspase-3 protein was observed by immunohistochemical method. Measurement data to mean ± standard deviation (x ± s) that the single-factor analysis of variance between groups, the results of P <0.05 was considered statistically significant. Results The structure of renal tissue in the control group was clear. The structure of the renal tissue in the exposure group decreased sharply. The pathological changes such as congestion and edema appeared 24 h after exposure, which aggravated with the prolongation of time. The glomeruli were also involved. The caspase-3 protein in the control group was not expressed much. The expression of caspase-3 protein in the membrane and cytoplasm of renal tubular epithelial cells in the cortex exposed 24 h after exposure was higher than that in the control group. The immunohistochemical score (IHS) The control group at each time point, the differences were statistically significant (P <0.05). Conclusion The high expression of caspase-3 protein suggests that it may be involved in the pathophysiology of PQ poisoning renal injury.