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采用大鼠离体肝细胞原代培养２４ｈ，并利用四氯化碳ＣＣｌ４造成急性肝细胞损伤模型，检定１５－甲基－前列腺素Ｆ２α（１５－Ｍｔ－ＰＧＦ２α）对肝细胞损伤的影响。结果表明：（１）１５－Ｍｔ－ＰＧＦ２α可显著降低中毒肝细胞脂质过氧化物水平，抑制肝细胞脂质过氧化，并降低谷丙转氨酶（ＧＰＴ）和谷草转氨酶（ＧＯＴ）水平，稳定脂质膜。（２）显著促进中毒肝细胞ＲＮＡ和ＤＮＡ的合成。（３）超微结构证实１５－Ｍｔ－ＰＧＦ２α能减轻ＣＣｌ４对肝细胞脂质膜，染色质，线粒体，内质网和核蛋白体的损害。
The rat hepatocytes were cultured in vitro for 24 hours. The acute liver injury model was induced by carbon tetrachloride (CCl4) and the effect of 15-methyl-prostaglandin F2α (15-Mt-PGF2α) on hepatocyte injury was evaluated. The results showed that: (1) 15-Mt-PGF2α could significantly reduce the level of lipid peroxides in hepatocytes, inhibit the lipid peroxidation of hepatocytes, decrease the levels of GPT and GOT, Plasma membrane. (2) Promote the synthesis of poisoned hepatocytes RNA and DNA significantly. (3) The ultrastructure confirmed that 15-Mt-PGF2α could reduce the damage of CCl4 on the lipid membrane, chromatin, mitochondria, endoplasmic reticulum and nucleoprotein of hepatocytes.