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绦虫病患者往往伴有脑囊虫病,前者易于诊断治疗,后者诊断较为困难,尤其是无临床症状的早期脑囊虫病患者更不易早期发现。我们用 IHA(间接血凝试验)方法,对治愈后的绦虫病患者进行动态观察。对 IHA 持续阳性者做进一步检查,查出无明显临床症状的脑囊虫病患者。这个方法对脑囊虫病的早期发现有一定意义。方法一、IHA 方法:从新鲜猪囊尾蚴中,吸出囊液,经透析后,离心,上清液为抗原,将1:10抗原用等量0.125%戊二醛醛化羊红细胞混合,37℃致敏30分钟后,洗去多余抗原,配成1%浓度。患者血清加在96孔 V 型血凝板上,以1:2～1:1024倍稀
Patients with taeniasis are often associated with cysticercosis, the former is easy to diagnose and treat, the latter is more difficult to diagnose, especially in patients without early symptoms of cerebral cysticercosis more difficult to early detection. We used IHA (Indirect Hemagglutination Test) method to dynamically observe the cured tapeworm patients. Continued positive IHA were further checked to detect no obvious clinical symptoms of cerebral cysticercosis patients. This method has some significance for the early detection of cysticercosis. Methods IHA method: fresh Cysticercus cellulosae, sucked out of the cyst fluid, after dialysis, centrifugation, the supernatant as antigen, the 1:10 antigen was mixed with 0.125% glutaraldehyde sheep red blood cells, 37 ℃ After sensitization 30 minutes, wash away the excess antigen, dubbed 1% concentration. Patient serum was added to a 96-well V-type plate, diluted 1: 2 to 1: 1024 times