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目的研究枳实提取物对实验性糖尿病小鼠肾脏抗氧化能力及胰岛形态的影响。方法将枳实提取物分成高、中、低剂量[5.1,3.4,1.7g/(kg.bw)]治疗糖尿病小鼠4周后,观察其一般状况、肾脏的抗氧化能力及胰岛形态变化。结果枳实高剂量组小鼠末期血糖为(20.37±5.25)mmol/L,明显低于糖尿病组的(25.74±2.99)mmol/L(P<0.05);枳实高剂量组谷胱甘肽(GSH)含量为〔(34.40±8.54)mg/(g.prot)〕,明显高于糖尿病组的〔(21.38±3.91)mg/(g.prot)〕(P<0.01);枳实高剂量组丙二醛(MDA)和NO含量分别为〔(0.89±0.37)nmol/(mg.prot)〕,〔(1.27±0.56)μmol/(g.prot)〕,明显低于糖尿病组的〔(1.46±0.39)nmol/(mg.prot)〕,〔(2.15±0.85)μmol/(g.prot)〕(P<0.01或P<0.05),光镜下枳实提取物治疗组胰岛细胞损伤程度较糖尿病组轻。结论枳实提取物能增强肾脏的抗氧化能力;保护胰岛组织细胞并降低胰岛细胞损伤。
Objective To study the effect of extracts of Citrus aurantium on the anti-oxidation ability and islet morphology of experimental diabetic mice. Methods The wolfberry extract was divided into high, medium and low dose [5.1, 3.4, 1.7 g/(kg.bw)] treated diabetic mice for 4 weeks. The general conditions, renal antioxidant capacity and islet morphology were observed. Results The blood glucose level in the high-dose group was (20.37±5.25) mmol/L, which was significantly lower than that in the diabetic group (25.74±2.99) mmol/L (P<0.05). The glutathione in the high-dose group was preserved. The GSH content was ((34.40±8.54) mg/(g.prot)), which was significantly higher than that of the diabetic group ((21.38±3.91) mg/(g.prot)) (P<0.01); Malondialdehyde (MDA) and NO levels were [(0.89±0.37) nmol/(mg.prot)] and [(1.27±0.56) μmol/(g.prot)], respectively, which were significantly lower than those in the diabetic group ((1.46). ± 0.39) nmol/(mg.prot)] and [(2.15±0.85) μmol/(g.prot)] (P<0.01 or P<0.05). The degree of islet cell damage was more severe in the curcuma extract treated group under the light microscope. The diabetes group is light. CONCLUSION: Citrus grandiflorum extract can enhance the antioxidant capacity of the kidney; protect islet cells and reduce islet cell damage.