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目的了解杭州市近年来自然水体中细菌耐药情况的基本特征及变化趋势。方法分别于2007、2008及2010年采集杭州市4个自然水体的水样,检测水样菌落形成单位(Colony-forming unit,CFU)以及氨苄青霉素耐药率,并用K-B法分析氨苄青霉素耐药菌株对其他抗生素的耐药情况。结果检测年份中,菌株对抗生素的耐药率呈现逐年递减的趋势:与2007年相比,2008年菌株对各种抗生素耐药情况的变化差异均无统计学意义;而2010年细菌对抗生素(氯霉素、环丙沙星、庆大霉素、四环素)的耐药率较2008年下降显著;细菌有从单纯耐药向多重耐药转变的趋势,2007、2008和2010年仅耐氨苄青霉素的菌株的比例分别为60.00%、39.02%和26.32%,呈下降趋势;而多重耐药细菌的比例增加明显,分别为17.50%、29.27%和48.68%。结论近年来,杭州市自然水体中细菌的耐药情况有所改善,但有单纯耐药向多重耐药转化的趋势。
Objective To understand the basic characteristics and trend of bacterial resistance in natural water in Hangzhou in recent years. Methods The water samples of four natural water bodies in Hangzhou were collected in 2007, 2008 and 2010, respectively. The colony-forming unit (CFU) and ampicillin resistance rates were detected. The antibiotic resistance of ampicillin Resistance to other antibiotics. Results In the detection year, the antibiotic resistance rate of the isolates showed a declining trend year by year. Compared with 2007, there was no significant difference in the resistance of isolates to various antibiotics in 2008. In 2010, Chloramphenicol, ciprofloxacin, gentamicin, tetracycline) decreased significantly compared with that in 2008. Bacteria had a tendency to change from simple drug resistance to multiple drug resistance. In 2007, 2008 and 2010, resistance to ampicillin Of the isolates were 60.00%, 39.02% and 26.32%, respectively. The proportion of multidrug-resistant bacteria increased significantly, which were 17.50%, 29.27% and 48.68% respectively. Conclusion In recent years, bacterial resistance in natural water bodies in Hangzhou has been improved, but there is a trend of simple drug-resistant transformation to multi-drug resistance.